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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNNC2 Polyclonal antibody

TNNC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15875-1-AP

Synonyms

TNNC2, troponin C type 2 (fast), Troponin C, skeletal muscle

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 15875-1-AP

    WB analysis of mouse skeletal muscle using 15875-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15875-1-AP (TNNC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat skeletal muscle using 15875-1-AP

    WB analysis of rat skeletal muscle using 15875-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15875-1-AP (TNNC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 15875-1-AP

    IHC staining of human skeletal muscle using 15875-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15875-1-AP (TNNC2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 15875-1-AP

    IHC staining of human skeletal muscle using 15875-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15875-1-AP (TNNC2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse skeletal muscle using 15875-1-AP

    IHC staining of mouse skeletal muscle using 15875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15875-1-AP (TNNC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 15875-1-AP

    IHC staining of mouse skeletal muscle using 15875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15875-1-AP (TNNC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 15875-1-AP is TNNC2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNNC2 fusion protein Ag8636 相同性解析による交差性が予測される生物種
Full Name troponin C type 2 (fast)
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC005323
Gene symbol TNNC2
Gene ID (NCBI) 7125
RRIDAB_2878194
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNNC2 antibody 15875-1-APDownload protocol
IHC protocol for TNNC2 antibody 15875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Rep

Transcriptional Dynamics of Hair-Bundle Morphogenesis Revealed with CellTrails.

Authors - Daniel C Ellwanger
View Article
humanWB

iScience

Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

Authors - Xiao-Hu Zhao
View Article