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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    WB analysis of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    WB analysis of human stomach using 13504-1-AP (same clone as 13504-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    IP experiment of mouse heart using 13504-1-AP (same clone as 13504-1-PBS)

    IP result of anti-TNNC1 (IP:13504-1-AP, 4ug; Detection:13504-1-AP 1:600) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    IHC staining of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13504-1-AP (TNNC1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using TNNC1 Polyclonal antibody (13504-1-AP)

    IHC staining of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13504-1-AP (TNNC1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TNNC1 Polyclonal antibody (13504-1-AP)

    IF Staining of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TNNC1 antibody (13504-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using TNNC1 Polyclonal antibody (13504-1-AP)

    FC experiment of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug TNNC1 Polyclonal antibody (13504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TNNC1 Polyclonal antibody, PBS Only

TNNC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13504-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

TN C, TNC, TN-C, TNNC, Troponin C

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13504-1-PBS targets TNNC1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4339

Product name: Recombinant human TNNC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-161 aa of BC030244

Sequence: MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

相同性解析による交差性が予測される生物種
Full Name troponin C type 1 (slow)
Calculated molecular weight 161 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC030244
Gene Symbol TNNC1
Gene ID (NCBI) 7134
RRIDAB_2256271
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP63316
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

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