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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNK1 Polyclonal antibody

TNK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14199-1-AP

Synonyms

CD38 negative kinase 1, TNK1

Validation Data Gallery

Applications:
  • WB analysis using 14199-1-AP

    WB analysis using 14199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14199-1-AP

    WB analysis using 14199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14199-1-AP

    WB analysis of mouse liver using 14199-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 14199-1-AP

    IHC staining of human prostate cancer using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 14199-1-AP

    IHC staining of human liver using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 14199-1-AP

    IHC staining of human liver using 14199-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14199-1-AP targets TNK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNK1 fusion protein Ag5402 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase, non-receptor, 1
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC035782
Gene symbol TNK1
Gene ID (NCBI) 8711
RRIDAB_2205700
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNK1 antibody 14199-1-APDownload protocol
IHC protocol for TNK1 antibody 14199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Thirty-Eight-Negative Kinase 1 Is a Mediator of Acute Kidney Injury in Experimental and Clinical Traumatic Hemorrhagic Shock.

Authors - Rebecca Halbgebauer
View Article
human,ratWB, IHC

3 Biotech

Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization.

Authors - Miaolong Tang
View Article