Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below | 
Product Information
68387-1-Ig targets TNIP1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human | 
| Cited Reactivity | human | 
| Host / Isotype | Mouse / IgG1 | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag8885 Product name: Recombinant human TNIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 287-636 aa of BC014008 Sequence: AGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ相同性解析による交差性が予測される生物種 | 
| Full Name | TNFAIP3 interacting protein 1 | 
| Calculated molecular weight | 72 kDa | 
| Observed molecular weight | 72 kDa | 
| GenBank accession number | BC014008 | 
| Gene Symbol | TNIP1 | 
| Gene ID (NCBI) | 10318 | 
| RRID | AB_3085105 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Protein G purification | 
| UNIPROT ID | Q15025 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Background Information
TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNIP1 antibody 68387-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 

