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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of K-562 using 67948-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using TNIK Monoclonal antibody (67948-1-Ig)

    WB analysis of human peripheral blood platelets using 67948-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TNIK Monoclonal antibody (67948-1-Ig)

    IHC staining of human kidney using 67948-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    IF Staining of A431 using 67948-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using TNIK Monoclonal antibody (67948-1-Ig)

    FC experiment of A431 using 67948-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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TNIK Monoclonal antibody

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67948-1-Ig
Clone No.1E9H3
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1E9H3, EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman peripheral blood platelets, K-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67948-1-Ig targets TNIK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TNIK fusion protein Ag30710 相同性解析による交差性が予測される生物種
Full Name TRAF2 and NCK interacting kinase
Calculated molecular weight 155 kDa
Observed molecular weight155~180 kDa
GenBank accession numberBC055427
Gene Symbol TNIK
Gene ID (NCBI) 23043
RRIDAB_2918700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Traf2- and Nck-interacting kinase (TNIK) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension (PMID: 24566388). TNIK is an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462).Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).

Protocols

Product Specific Protocols
WB protocol for TNIK antibody 67948-1-IgDownload protocol
IHC protocol for TNIK antibody 67948-1-IgDownload protocol
IF protocol for TNIK antibody 67948-1-IgDownload protocol
FC protocol for TNIK antibody 67948-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Mol Biosci

Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data

Authors - Ya-Jun Deng
View Article