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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFSF15 Recombinant monoclonal antibody (87367-3-RR)

    WB analysis using 87367-3-RR (same clone as 87367-3-PBS)

    Transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 87367-3-RR (TNFSF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 87367-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using TNFSF15 Recombinant monoclonal antibody (87367-3-RR)

    IF Staining of Transfected HEK-293 using 87367-3-RR (same clone as 87367-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using TNFSF15 antibody (87367-3-RR, Clone: 250983F1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 87367-3-PBS in a different storage buffer formulation.

  • Affinity of 87367-3-RR

    Affinity of 87367-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87367-3-RR against Mouse TNFSF15 were performed. The affinity constant is below 1 pM.

Mouse TNFSF15 Recombinant monoclonal antibody, PBS Only

TNFSF15 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87367-3-PBS
Clone No.250983F1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Tl1, Tl1, Vegi, TNF15, TNFSF 15, Vegi

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87367-3-PBS targets TNFSF15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg5273

Product name: Recombinant Mouse TNFSF15 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 61-252 aa of /

Sequence: AGQLRVPGKDCMLRAITEERSEPSPQQVYSPPRGKPRAHLTIKKQTPAPHLKNQLSALHWEHDLGMAFTKNGMKYINKSLVIPESGDYFIYSQITFRGTTSVCGDISRGRRPNKPDSITVVITKVADSYPEPARLLTGSKSVCEISNNWFQSLYLGAMFSLEEGDRLMVNVSDISLVDYTKEDKTFFGAFLL

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 15
Calculated molecular weight28 kDa
Observed molecular weight35 kDa
GenBank accession number/
Gene Symbol Tnfsf15
Gene ID (NCBI) 326623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5UBV8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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