Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, MDA-MB-453s cells, PC-3 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below | 
| WB | See 1 publications below | 
Product Information
12537-1-AP targets TNFSF12 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Cited Reactivity | human, mouse | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag3242 Product name: Recombinant human TNFSF12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of BC019047 Sequence: MAARRSQRRRGRRGEPGTALLVPLALGLGLALACLGLLLAVVSLGSRASLSAQEPAQEELVAEEDQDPSELNPQTEESQDPAPFLNRLVRPRRSAPKGRKTRARRAIAAHYEVHPRPGQDGAQADGGYTTCLRP相同性解析による交差性が予測される生物種 | 
| Full Name | tumor necrosis factor (ligand) superfamily, member 12 | 
| Calculated molecular weight | 330 aa, 37 kDa | 
| Observed molecular weight | 22 kDa | 
| GenBank accession number | BC019047 | 
| Gene Symbol | TNFSF12 | 
| Gene ID (NCBI) | 8742 | 
| RRID | AB_2206217 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | O43508 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFSF12 antibody 12537-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 



