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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TNFSF12 Polyclonal antibody

TNFSF12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12537-1-AP

Synonyms

APO3 ligand, APO3L, DR3LG, TNFSF12, TWEAK

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 12537-1-AP

    WB analysis of HEK-293 using 12537-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12537-1-AP (TNFSF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 12537-1-AP

    WB analysis of MDA-MB-453s using 12537-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12537-1-AP (TNFSF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 12537-1-AP

    WB analysis of PC-3 using 12537-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12537-1-AP (TNFSF12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12537-1-AP targets TNFSF12 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFSF12 fusion protein Ag3242 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 12
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC019047
Gene symbol TNFSF12
Gene ID (NCBI) 8742
RRIDAB_2206217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TNFSF12 antibody 12537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols