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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    IF Staining of human tonsillitis using 98112-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    IF Staining of human tonsillitis using 98112-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TNFRSF9/CD137 antibody (98112-1-RR, Clone: 241344F3 ) at dilution of 1:180 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated or Con-A-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibo (98112-1-RR)

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated (black) or Con-A-stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98112-1-RR

    Affinity and Kinetic Characterization of 98112-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98112-1-RR against Human TNFRSF9/CD137 were performed. The affinity constant is 10.3 nM.

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Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

TNFRSF9/CD137 Uni-rAbTM Recombinant Antibody for IF-P, FC
Cat No. 98112-1-RR
Clone No.241344F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, FC

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
PE
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inCon-A-stimulated human PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98112-1-RR targets TNFRSF9/CD137 in IF-P, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 9
Calculated molecular weight 28 kDa
GenBank accession numberBC006196
Gene Symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
RRIDAB_3672259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07011
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

Protocols

Product Specific Protocols
IF protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
FC protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols