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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFRSF13B/CD267 Polyclonal antibody (21520-1-AP)

    WB analysis using 21520-1-AP

    IM-9 cells were subjected to SDS PAGE followed by western blot with 21520-1-AP (TNFRSF13B/CD267 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TNFRSF13B Polyclonal antibody (21520-1-AP)

    WB analysis of K-562 using 21520-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21520-1-AP (TNFRSF13B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

TNFRSF13B/CD267 Polyclonal antibody

TNFRSF13B/CD267 Polyclonal Antibody for WB, ELISA
Cat No. 21520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD267, TNFRSF13B, TACI, CVID

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inIM-9 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21520-1-AP targets TNFRSF13B/CD267 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16097

Product name: Recombinant human TNFRSF13B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 133-247 aa of BC109392

Sequence: LVAVACFLKKRGDPCSCQPRSRPRQSPAKSSQDHAMEAGSPVSTSPEPVETCSFCFPECRAPTQESAVTPGTPDPTCAGRWGCHTRTTVLQPCPHIPDSGLGIVCVPAQEGGPGA

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 13B
Calculated molecular weight 293 aa, 32 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC109392
Gene Symbol TNFRSF13B/CD267
Gene ID (NCBI) 23495
RRIDAB_10888631
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14836
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B), also known as CD267 or TACI, is a transmembrane protein of the TNF receptor superfamily found predominantly on B cells (PMID: 10920230). TACI is also expressed on activated T cells, myeloma cells, and expressed by monocytes intracellularly at a low level (PMID: 11429548; 16825497). TNFRSF13B is a receptor for TNFSF13/APRIL/CD256 and TNFSF13B/BAFF/CD257 and mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-κB and AP-1 (PMID: 10956646; 10973284; 9311921). It is involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.

Protocols

Product Specific Protocols
WB protocol for TNFRSF13B/CD267 antibody 21520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols