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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RANK/TNFRSF11A Polyclonal antibody (32528-1-AP)

    WB analysis using 32528-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32528-1-AP (TNFRSF11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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RANK/TNFRSF11A Polyclonal antibody

RANK/TNFRSF11A Polyclonal Antibody for WB, ELISA
Cat No. 32528-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

TNFRSF11A, TNF receptor superfamily member 11A , Receptor activator of NF-KB, RANK, Osteoclast differentiation factor receptor (ODFR)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC2C12 cells, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32528-1-AP targets RANK/TNFRSF11A in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Tumor necrosis factor receptor superfamily, member 11a
Calculated molecular weight68 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_001271235.1
Gene Symbol Tnfrsf11a
Gene ID (NCBI) 498206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDF1M8Z6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A, also known as RANK, CD265, and ODFR) is the signaling receptor essential for osteoclast differentiation factor-mediated osteoclastogenesis and is involved in the regulation of interactions between T-cells and dendritic cells (PMID: 9878548). The RANK/RANKL/OPG system is a key regulator in bone resorption, and its dysregulation can lead to pathological bone loss (PMID: 37893470).

Protocols

Product Specific Protocols
WB protocol for RANK/TNFRSF11A antibody 32528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols