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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    WB analysis of HeLa using 15320-1-AP (same clone as 15320-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    WB analysis of L02 using 15320-1-AP (same clone as 15320-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human placenta using 15320-1-AP (same clone as 15320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human placenta using 15320-1-AP (same clone as 15320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human brain using 15320-1-AP (same clone as 15320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IHC staining of human brain using 15320-1-AP (same clone as 15320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TNFAIP1 Polyclonal antibody (15320-1-AP)

    IF Staining of HeLa using 15320-1-AP (same clone as 15320-1-PBS)

    Immunofluorescent analysis of HeLa cells using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15320-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15320-1-PBS targets TNFAIP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNFAIP1 fusion protein Ag0220 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 1 (endothelial)
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC001949
Gene Symbol TNFAIP1
Gene ID (NCBI) 7126
RRIDAB_10644288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13829
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.