Validation Data Gallery

Filter: AllWBIHCIF

WB analysis using 67710-1-Ig (same clone as 67710-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 67710-1-Ig (same clone as 67710-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 67710-1-Ig (same clone as 67710-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 67710-1-Ig (same clone as 67710-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using TNC/Tenascin-C antibody (67710-1-Ig, Clone: 1F7G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67710-1-PBS in a different storage buffer formulation.

TNC/Tenascin-C Monoclonal antibody, PBS Only

TNC/Tenascin-C Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67710-1-PBS

Clone No.1F7G8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67710-1-PBS targets TNC/Tenascin-C in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag21412 Product name: Recombinant human TNC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1852-2201 aa of BC151843 Sequence: YALTDLEPATEYTLRIFAEKGPQKSSTITAKFTTDLDSPRDLTATEVQSETALLTWRPPRASVTGYLLVYESVDGTVKEVIVGPDTTSYSLADLSPSTHYTAKIQALNGPLRSNMIQTIFTTIGLLYPFPKDCSQAMLNGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA 相同性解析による交差性が予測される生物種
Full Name	tenascin C
Calculated molecular weight	2201 aa, 241 kDa
Observed molecular weight	260 kDa
GenBank accession number	BC151843
Gene Symbol	Tenascin C
Gene ID (NCBI)	3371
RRID	AB_2882900
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P24821
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67710-1-Ig (same clone as 67710-1-PBS)

WB analysis of rat cerebellum using 67710-1-Ig (same clone as 67710-1-PBS)

WB analysis of HEK-293 using 67710-1-Ig (same clone as 67710-1-PBS)

IHC staining of human breast cancer using 67710-1-Ig (same clone as 67710-1-PBS)