Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 21348-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21348-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 21348-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 21348-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21348-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 21348-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 21348-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 21348-1-AP
Immunofluorescent analysis of HepG2 cells using 21348-1-AP (TMX4 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

TMX4 Polyclonal antibody

TMX4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21348-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ475/PRO938, TXNDC13, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13, PDIA14

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, A375 cells, human heart tissue, L02 cells
Positive IHC detected in	human brain tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

21348-1-AP targets TMX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15696 Product name: Recombinant human TMX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-349 aa of BC033787 Sequence: PAWCSYVFFVIATLVFGLFMGLVLVVISECFYVPLPRHLSERSEQNRRSEEAHRAEQLQDAEEEKDDSNEEENKDSLVDDEEEKEDLGDEDEAEEEEEEDNLAAGVDEERSEANDQGPPREDGVTREEVEPEEAEEGISEQPCPADTEVVEDSLRQRKSQHADKGL 相同性解析による交差性が予測される生物種
Full Name	thioredoxin-related transmembrane protein 4
Calculated molecular weight	349 aa, 39 kDa
Observed molecular weight	40-45 kDa
GenBank accession number	BC033787
Gene Symbol	TMX4
Gene ID (NCBI)	56255
RRID	AB_10733353
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1E5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.

Protocols

Product Specific Protocols
FC protocol for TMX4 antibody 21348-1-AP	Download protocol
IF protocol for TMX4 antibody 21348-1-AP	Download protocol
IHC protocol for TMX4 antibody 21348-1-AP	Download protocol
WB protocol for TMX4 antibody 21348-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB,IF	Nat Commun TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress Authors - Marika K Kucińska View Article
human	WB	J Proteome Res Mapping of the N-linked glycoproteome of human spermatozoa. Authors - Wang Gaigai G View Article
mouse	WB	JCI Insight Protein disulfide isomerase is essential for spermatogenesis in mice Authors - Yaqiong Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 21348-1-AP

WB analysis of mouse brain using 21348-1-AP

WB analysis of mouse brain using 21348-1-AP

WB analysis of human heart using 21348-1-AP

WB analysis of L02 using 21348-1-AP

WB analysis of A375 using 21348-1-AP

IHC staining of human brain using 21348-1-AP

IHC staining of human brain using 21348-1-AP

IHC staining of human testis using 21348-1-AP

IHC staining of human testis using 21348-1-AP

IHC staining of human testis using 21348-1-AP

IHC staining of human testis using 21348-1-AP

IF Staining of HepG2 using 21348-1-AP

TMX4 Polyclonal antibody

TMX4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress

Mapping of the N-linked glycoproteome of human spermatozoa.

Protein disulfide isomerase is essential for spermatogenesis in mice