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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

TMPRSS3 Polyclonal antibody

TMPRSS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17953-1-AP

Synonyms

DFNB10, DFNB8, ECHOS1, Serine protease TADG 12, TADG12, TMPRSS3

Validation Data Gallery

Applications:
  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 17953-1-AP

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 17953-1-AP

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin cancer using 17953-1-AP

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17953-1-AP is TMPRSS3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMPRSS3 fusion protein Ag12435 相同性解析による交差性が予測される生物種
Full Name transmembrane protease, serine 3
Calculated molecular weight 453 aa, 49 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC074846
Gene symbol TMPRSS3
Gene ID (NCBI) 64699
RRIDAB_2878470
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMPRSS3 antibody 17953-1-APDownload protocol
IHC protocol for TMPRSS3 antibody 17953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Gene Med

TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.

Authors - Zhi-Wen Zhang
  • KD Validated
View Article
mouseIF

Mol Ther Methods Clin Dev

Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea

Authors - Ksenia A Aaron
  • KO Validated
View Article