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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    WB analysis of mouse skin using 17953-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human cervical cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using TMPRSS3 Polyclonal antibody (17953-1-AP)

    IHC staining of human skin cancer using 17953-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17953-1-AP (TMPRSS3 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO Validated

TMPRSS3 Polyclonal antibody

TMPRSS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17953-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

TADG12, Serine protease TADG-12, ECHOS1, EC:3.4.21.-, DFNB8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17953-1-AP targets TMPRSS3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12435

Product name: Recombinant human TMPRSS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 262-453 aa of BC074846

Sequence: LYLPKSWTIQVGLVSLLDNPAPSHLVEKIVYHSKYKPKRLGNDIALMKLAGPLTFNEMIQPVCLPNSEENFPDGKVCWTSGWGATEDGGDASPVLNHAAVPLISNKICNHRDVYGGIISPSMLCAGYLTGGVDSCQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYTRVTSFLDWIHEQMERDLKT

相同性解析による交差性が予測される生物種
Full Name transmembrane protease, serine 3
Calculated molecular weight 453 aa, 49 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC074846
Gene Symbol TMPRSS3
Gene ID (NCBI) 64699
RRIDAB_2878470
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP57727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TMPRSS3 antibody 17953-1-APDownload protocol
WB protocol for TMPRSS3 antibody 17953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Gene Med

TMPRSS3 regulates cell viability and apoptosis processes of HEI-OC1 cells via regulation on circ-Slc4a2, miR-182, and AKT cascade.

Authors - Zhi-Wen Zhang
  • KD Validated
View Article
mouseIF

Mol Ther Methods Clin Dev

Selection of viral capsids and promoters affects the efficacy of rescue of Tmprss3-deficient cochlea

Authors - Ksenia A Aaron
  • KO Validated
View Article
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