Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, human heart tissue, human kidney tissue, human lung tissue, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
12521-1-AP targets TMOD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3215 Product name: Recombinant human TMOD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC020542 Sequence: MALPFRKDLEKYKDLDEDELLGNLSETELKQLETVLDDLDPENALLPAGFRQKNQTSKSTTGPFDREHLLSYLEKEALEHKDREDYVPYTGEKKGKIFIPKQKPVQTFTEEKVSLDPELEEALTSASDTELCDLAAILGMHNLITNTKFCNIMGSSNGVDQEHFSNVVKGEKILPVFDEPPNPTNVEESLKRTKENDAHLVEVNLNNIKNIPIPTLKDFAKALETNTHVKCFSLAATRSNDPVATAFAEMLKVNKTLKSLNVESNFITGVGILALIDALRDNETLAELKIDNQRQQLGTAVELEMAKMLEENTNILKFGYQFTQQGPRTRAANAITKNNDLVRKRRVEGDHQ 相同性解析による交差性が予測される生物種 |
| Full Name | tropomodulin 3 (ubiquitous) |
| Calculated molecular weight | 352 aa, 40 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC020542 |
| Gene Symbol | TMOD3 |
| Gene ID (NCBI) | 29766 |
| RRID | AB_2205324 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYL9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tropomodulin 3(TMOD3) belongs to the tropomodulin family, which controls cytoskeletal dynamics by regulating the end-capping of actin filaments. TMOD3 bridges the gap between structural integrity and signaling cascades. TMOD3 regulates cytoskeletal architecture and is implicated in cell adhesion, migration, and mechanotransduction.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| IHC protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| IP protocol for TMOD3 antibody 12521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carcinogenesis MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3. | ||
Comb Chem High Throughput Screen Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma | ||
Drug Resist Updat TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4 |