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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMOD1 Polyclonal antibody (10145-1-AP)

    WB analysis using 10145-1-AP (same clone as 10145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10145-1-AP (TMOD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMOD1 Polyclonal antibody (10145-1-AP)

    WB analysis using 10145-1-AP (same clone as 10145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10145-1-AP (TMOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TMOD1 Polyclonal antibody (10145-1-AP)

    IHC staining of mouse heart using 10145-1-AP (same clone as 10145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TMOD1 Polyclonal antibody (10145-1-AP)

    IHC staining of mouse heart using 10145-1-AP (same clone as 10145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10145-1-AP (TMOD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using TMOD1 Polyclonal antibody (10145-1-AP)

    IF Staining of mouse heart using 10145-1-AP (same clone as 10145-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using TMOD1 Polyclonal antibody (10145-1-AP)

    IF Staining of mouse heart using 10145-1-AP (same clone as 10145-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using TMOD1 antibody (10145-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10145-1-PBS in a different storage buffer formulation.

TMOD1 Polyclonal antibody, PBS Only

TMOD1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 10145-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

D9S57E, E-Tmod, TMOD, tropomodulin 1, Tropomodulin-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10145-1-PBS targets TMOD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0192

Product name: Recombinant human TMOD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 105-359 aa of BC002660

Sequence: PVLESVTLEPELEEALANASDAELCDIAAILGMHTLMSNQQYYQALSSSSIMNKEGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPKLEEVNLNNIRNIPIPTLKAYAEALKENSYVKKFSIVGTRSNDPVAYALAEMLKENKVLKTLNVESNFISGAGILRLVEALPYNTSLVEMKIDNQSQPLGNKVEMEIVSMLEKNATLLKFGYHFTQQGPRLRASNAMMNNNDLVRKRRLADLTGPIIPKCRSGV

相同性解析による交差性が予測される生物種
Full Name tropomodulin 1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC002660
Gene Symbol TMOD1
Gene ID (NCBI) 7111
RRIDAB_2205316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP28289
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Actin filaments control cell morphology. The length of these filaments is regulated in muscle and nonmuscle cell types by tropomodulins 1-4 (Tmod1-4), a family of proteins that cap the pointed ends of actin filaments. The molecular weight of Tmod 1 is about 40 kDa.

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