Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, HUVEC cells, Raji cells, MDCK cells |
| Positive IF-P detected in | rat small intestine tissue, mouse small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84130-6-RR targets TMIGD1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
| Tested Reactivity | human, mouse, rat, dog |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag25691 Product name: Recombinant human TMIGD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 133-207 aa of BC137201 Sequence: VEEGSNVKLVCNVKANPQAQMMWYKNSSLLDLEKSRHQIQQTSESFQLSITKVEKPDNGTYSCIAKSSLKTESLD 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane and immunoglobulin domain containing 1 |
| Calculated molecular weight | 262 aa, 29 kDa |
| Observed molecular weight | 45 kDa, 29 kDa |
| GenBank accession number | BC137201 |
| Gene Symbol | TMIGD1 |
| Gene ID (NCBI) | 388364 |
| RRID | AB_3743523 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6UXZ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMIGD1 antibody 84130-6-RR | Download protocol |
| WB protocol for TMIGD1 antibody 84130-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |