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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMIGD1 Polyclonal antibody (27174-1-AP)

    WB analysis using 27174-1-AP (same clone as 27174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    WB analysis of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27174-1-AP (TMIGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IHC staining of mouse kidney using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IHC staining of human colon using 27174-1-AP (same clone as 27174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27174-1-AP (TMIGD1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IF Staining of rat small intestine using 27174-1-AP (same clone as 27174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using TMIGD1 Polyclonal antibody (27174-1-AP)

    IF Staining of mouse small intestine using 27174-1-AP (same clone as 27174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using TMIGD1 antibody (27174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27174-1-PBS in a different storage buffer formulation.

TMIGD1 Polyclonal antibody, PBS Only

TMIGD1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 27174-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TMIGD, Transmembrane and immunoglobulin domain-containing protein 1, UNQ9372/PRO34164

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27174-1-PBS targets TMIGD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25691

Product name: Recombinant human TMIGD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 133-207 aa of BC137201

Sequence: VEEGSNVKLVCNVKANPQAQMMWYKNSSLLDLEKSRHQIQQTSESFQLSITKVEKPDNGTYSCIAKSSLKTESLD

相同性解析による交差性が予測される生物種
Full Name transmembrane and immunoglobulin domain containing 1
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 45 kDa, 29 kDa
GenBank accession numberBC137201
Gene Symbol TMIGD1
Gene ID (NCBI) 388364
RRIDAB_2880786
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXZ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMIGD1 (Transmembrane and immunoglobulin domain containing 1) is involved in various cellular processes such as regulation of cell structure, cell-cell adhesion, and cellular movement. It protects cells from oxidative- and nutrient-deprivation-induced cell injury by stabilizing the transepithelial electric resistance and permeability of renal epithelial cells. TMIGD1 has a calculated molecular mass of 29 kDa, and can be detected as 45 kDa due to the N-glycosylation (PMID: 26342724).

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