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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMF1-Specific Polyclonal antibody (19728-1-AP)

    WB analysis using 19728-1-AP (same clone as 19728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using TMF1-Specific Polyclonal antibody (19728-1-AP)

    WB analysis of BxPC-3 using 19728-1-AP (same clone as 19728-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TMF1-Specific Polyclonal antibody (19728-1-AP)

    WB analysis of L02 using 19728-1-AP (same clone as 19728-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IP experiment of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    IP result of anti-TMF1-Specific (IP:19728-1-AP, 4ug; Detection:19728-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMF1-Specific Polyclonal antibody (19728-1-AP)

    IF Staining of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

TMF1-Specific Polyclonal antibody, PBS Only

TMF1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19728-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19728-1-PBS targets TMF1-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name TATA element modulatory factor 1
Calculated molecular weight 123 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberNM_007114
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_10667009
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP82094
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

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