Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, MCF-7 cells, BT-549 cells, Caco-2 cells, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86837-1-RR targets TMEM97 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34803 Product name: Recombinant human TMEM97 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-69 aa of BC045655 Sequence: MTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 97 |
| Observed molecular weight | 19-20 kDa |
| GenBank accession number | BC045655 |
| Gene Symbol | TMEM97 |
| Gene ID (NCBI) | 27346 |
| RRID | AB_3745153 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5BJF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transmembrane protein 97 (TMEM97) is an endoplasmic reticulum (ER)-residing protein that belongs to the EXPERA domain-containing family whose function is overall understudied. TMEM97 is also linked to cholesterol homeostasis, Niemann-Pick disease, Alzheimer disease, and neuropathic pain. Intriguingly, TMEM97 was recently unveiled to be the sigma-2 receptor (S2R), an enigmatic drug-binding site that had been pharmaceutically targeted for decades in treating psychotic disorders.(PMID: 39995975, PMID: 38290642)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM97 antibody 86837-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |