Validation Data Gallery
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Tested Applications
| Positive WB detected in | Caco-2 cells, HEK-293T cells, HeLa cells, mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
26444-1-AP targets TMEM97 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23917 Product name: Recombinant human TMEM97 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-69 aa of BC045655 Sequence: MTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 97 |
| Observed molecular weight | 19-20 kDa |
| GenBank accession number | BC045655 |
| Gene Symbol | TMEM97 |
| Gene ID (NCBI) | 27346 |
| RRID | AB_2880515 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5BJF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM97 antibody 26444-1-AP | Download protocol |
| WB protocol for TMEM97 antibody 26444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. | ||
ACS Chem Neurosci Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding
| ||
J Bioenerg Biomembr Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling. | ||
Life Sci Alliance The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth | ||
Cell Biosci PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression |
