Validation Data Gallery

Filter: AllWBIHC

WB analysis using 26444-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 26444-1-AP (TMEM97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 26444-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 26444-1-AP (TMEM97 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 26444-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 26444-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 26444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 26444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26444-1-AP (TMEM97 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

TMEM97 Polyclonal antibody

TMEM97 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26444-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

MAC30, Protein MAC30, TMEM97, transmembrane protein 97

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Caco-2 cells, mouse testis tissue
Positive IHC detected in	mouse testis tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 1 publications below

Product Information

26444-1-AP targets TMEM97 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TMEM97 fusion protein Ag23917 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 97
Observed molecular weight	19-20 kDa
GenBank accession number	BC045655
Gene Symbol	TMEM97
Gene ID (NCBI)	27346
RRID	AB_2880515
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5BJF2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM97 antibody 26444-1-AP	Download protocol
IHC protocol for TMEM97 antibody 26444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Chem Biol A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes. Authors - Yu-Shiuan Cheng View Article
mouse	WB	ACS Chem Neurosci Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding Authors - Robert C C Mercer KD Validated View Article
human,mouse		Cell Signal TMEM97 ablation aggravates oxidant-induced retinal degeneration. Authors - Hongtao Shen KO Validated View Article
human	WB	J Bioenerg Biomembr Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling. Authors - Yu Zhang View Article
mouse	WB	Life Sci Alliance The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth Authors - Marina Badenes View Article
human	WB,IHC	Cell Biosci PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression Authors - Liang Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26444-1-AP

WB analysis of mouse testis using 26444-1-AP

IHC staining of mouse testis using 26444-1-AP

IHC staining of mouse testis using 26444-1-AP

IHC staining of human liver using 26444-1-AP

IHC staining of human liver using 26444-1-AP

TMEM97 Polyclonal antibody

TMEM97 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.

Sigma Receptor Ligands Are Potent Antiprion Compounds that Act Independently of Sigma Receptor Binding

TMEM97 ablation aggravates oxidant-induced retinal degeneration.

Meningioma-associated protein 30 accelerates the proliferation and invasion of hepatocellular carcinoma by modulating Wnt/GSK-3β/β-catenin signaling.

The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression