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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis using 25091-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis of MDA-MB-453s using 25091-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TMEM87A Polyclonal antibody (25091-1-AP)

    WB analysis of PC-3 using 25091-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25091-1-AP (TMEM87A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human breast cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human prostate cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM87A Polyclonal antibody (25091-1-AP)

    IHC staining of human prostate cancer using 25091-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25091-1-AP (TMEM87A Antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TMEM87A Polyclonal antibody

TMEM87A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25091-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein 87A, TM87A, PSEC0094, Elkin1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, MDA-MB-453s cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25091-1-AP targets TMEM87A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM87A fusion protein Ag18715 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 87A
Calculated molecular weight 555 aa, 63 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC005335
Gene Symbol TMEM87A
Gene ID (NCBI) 25963
RRIDAB_2879892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBN3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM87A antibody 25091-1-APDownload protocol
IHC protocol for TMEM87A antibody 25091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols