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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM77 Polyclonal antibody (23182-1-AP)

    WB analysis using 23182-1-AP (same clone as 23182-1-PBS)

    K-562 cells lysate was subjected to SDS PAGE followed by western blot with 23182-1-AP (TMEM77 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human ovarian cancer using 23182-1-AP (same clone as 23182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 23182-1-AP (TMEM77 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human heart using 23182-1-AP (same clone as 23182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23182-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM77 Polyclonal antibody (23182-1-AP)

    IHC staining of human heart using 23182-1-AP (same clone as 23182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23182-1-AP (TMEM77 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23182-1-PBS in a different storage buffer formulation.

TMEM77 Polyclonal antibody, PBS Only

TMEM77 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 23182-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

DNA damage-regulated autophagy modulator protein 2, DRAM2, PRO180, UNQ154/PRO180

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23182-1-PBS targets TMEM77 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19711

Product name: Recombinant human TMEM77 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 170-266 aa of BC047025

Sequence: ALSMLTCSSVLHSGNFGTDLEQKLHWNPEDKGYVLHMITTAAEWSMSFSFFGFFLTYIRDFQKISLRVEANLHGLTLYDTAPCPINNERTRLLSRDI

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 77
Calculated molecular weight 266 aa, 30 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC047025
Gene Symbol TMEM77
Gene ID (NCBI) 128338
RRIDAB_2879225
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ6UX65
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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