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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of LNCaP using 67773-1-Ig (same clone as 67773-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of L02 using 67773-1-Ig (same clone as 67773-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TMEM74 Monoclonal antibody (67773-1-Ig)

    WB analysis of HEK-293 using 67773-1-Ig (same clone as 67773-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67773-1-PBS in a different storage buffer formulation.

TMEM74 Monoclonal antibody, PBS Only

TMEM74 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67773-1-PBS
Clone No.2H1B7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

FLJ30668, TMEM74, transmembrane protein 74

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67773-1-PBS targets TMEM74 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag22359

Product name: Recombinant human TMEM74 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-187 aa of BC030710

Sequence: MELHYLAKKSNQADLCDARDWSSRGLPGDQADTAATRAALCCQKQCASTPRATEMEGSKLSSSPASPSSSLQNSTLQPDAFPPGLLHSGNNQITAERKVCNCCSQELETSFTYVDKNINLEQRNRSSPSAKGHNHPGELGWENPNEWSQEAAISLISEEEDDTSSEATSSGKSIDYGFISAILFLVT

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 74
Calculated molecular weight 305 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC030710
Gene Symbol TMEM74
Gene ID (NCBI) 157753
RRIDAB_2918538
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ96NL1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)

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