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Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using TMEM70 Monoclonal antibody (60195-1-Ig)

    WB analysis of A2780 using 60195-1-Ig (same clone as 60195-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 60195-1-Ig (TMEM70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TMEM70 Monoclonal antibody (60195-1-Ig)

    WB analysis of HeLa using 60195-1-Ig (same clone as 60195-1-PBS)

    WB result of TMEM70 antibody (60195-1-Ig; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM70 transfected HeLa cells. This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM70 Monoclonal antibody (60195-1-Ig)

    IHC staining of human liver using 60195-1-Ig (same clone as 60195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM70 Monoclonal antibody (60195-1-Ig)

    IHC staining of human liver using 60195-1-Ig (same clone as 60195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using TMEM70 Monoclonal antibody (60195-1-Ig)

    IHC staining of human pancreas using 60195-1-Ig (same clone as 60195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using TMEM70 Monoclonal antibody (60195-1-Ig)

    IHC staining of human pancreas using 60195-1-Ig (same clone as 60195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60195-1-Ig(TMEM70 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60195-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TMEM70 Monoclonal antibody, PBS Only

TMEM70 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60195-1-PBS
Clone No.2C11C3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TMEM70, transmembrane protein 70

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60195-1-PBS targets TMEM70 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMEM70 fusion protein Ag16947 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 70
Calculated molecular weight 260 aa, 29 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002748
Gene Symbol TMEM70
Gene ID (NCBI) 54968
RRIDAB_10896316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUB7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication (PMID:21147908) identified TMEM70 gene defect as a pan-ethnic disorder and further redefined it as the most common cause of nuclear-origin ATP synthase deficiency.