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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM64 Polyclonal antibody (21274-1-AP)

    WB analysis using 21274-1-AP (same clone as 21274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21274-1-AP (TMEM64 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM64 Polyclonal antibody (21274-1-AP)

    IHC staining of human prostate cancer using 21274-1-AP (same clone as 21274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 21274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TMEM64 Polyclonal antibody (21274-1-AP)

    IHC staining of human prostate cancer using 21274-1-AP (same clone as 21274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 21274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM64 Polyclonal antibody (21274-1-AP)

    IHC staining of mouse brain using 21274-1-AP (same clone as 21274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21274-1-AP (TMEM64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21274-1-PBS in a different storage buffer formulation.

TMEM64 Polyclonal antibody, PBS Only

TMEM64 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Transmembrane protein 64

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21274-1-PBS targets TMEM64 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15753

Product name: Recombinant human TMEM64 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-380 aa of BC113828

Sequence: DVIAEQSVSGYFVFCLQIIISIGLMFYVVHRAQVELNAAIVACEMELKSSLVKGNQPNTSGSSFYNKRTLTFSGGGINVV

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 64
Calculated molecular weight 380 aa, 40 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC113828
Gene Symbol TMEM64
Gene ID (NCBI) 169200
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6YI46
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tmem64 (transmembrane protein 64) is a positive modulator of osteoclast differentiation by SERCA2-dependent Ca2+ signaling (PMID: 23395171). Altered Levels of mRNAs for Calcium-Binding/Associated Proteins, Annexin A1, S100A4, and TMEM64 are associated with osteoporosis in peripheral blood mononuclear cells (PMID: 31814858). Tmem64 is involved in the metastatic progression of prostate cancer cells by regulating Wnt3a secretion (PMID: 31289498).

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