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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    IF Staining of HepG2 using 83656-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TMEM59 antibody (83656-3-RR, Clone: 240457A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using TMEM59 Recombinant antibody (83656-3-RR)

    FC experiment of HepG2 using 83656-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM59 Recombinant antibody (83656-3-RR, Clone:240457A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83656-3-RR

    Affinity and Kinetic Characterization of 83656-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83656-3-RR against Human TMEM59 were performed. The affinity constant is below 1 pM.

TMEM59 Recombinant monoclonal antibody

TMEM59 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83656-3-RR
Clone No.240457A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

UNQ169/PRO195, Liver membrane-bound protein, HSPC001, C1orf8, 240457A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83656-3-RR targets TMEM59 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1968

Product name: Recombinant human TMEM59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-324 aa of BC016374

Sequence: MAAPKGSLWVRTQLGLPPLLLLTMALAGGSGTASAEAFDSVLGDTASCHRACQLTYPLHTYPKEEELYACQRGCRLFSICQFVDDGIDLNRTKLECESACTEAYSQSDEQYACHLGCQNQLPFAELRQEQLMSLMPKMHLLFPLTLVRSFWSDMMDSAQSFITSSWTFYLQADDGKIVIFQSKPEIQYAPHLEQEPTNLRESSLSKMSSDLQMRNSQAHRNFLEDGESDGFLRCLSLNSGWILTTTLVLSVMVLLWICCATVATAVEQYVPSEKLSIYGDLEFMNEQKLNRYPASSLVVVRSKTEDHEEAGPLPTKVNLAHSEI

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 59
Calculated molecular weight 36 kDa
GenBank accession numberBC016374
Gene Symbol TMEM59
Gene ID (NCBI) 9528
RRIDAB_3671264
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM59 is a regulator of autophagy in response to bacterial infection. Overexpression of this protein has been found to promote apoptosis in a glioma cell line. Alternative splicing of TMEM59 results in multiple transcript variants.

Protocols

Product Specific Protocols
IF protocol for TMEM59 antibody 83656-3-RRDownload protocol
FC protocol for TMEM59 antibody 83656-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols