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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TMEM41B Polyclonal antibody (29270-1-AP)

    WB analysis of HeLa using 29270-1-AP (same clone as 29270-1-PBS)

    WB result of TMEM41B antibody (29270-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM41B transfected HeLa cells. This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM41B Polyclonal antibody (29270-1-AP)

    WB analysis using 29270-1-AP (same clone as 29270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29270-1-AP (TMEM41B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of mouse lung using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of mouse lung using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of mouse testis using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of mouse testis using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of human lung using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TMEM41B Polyclonal antibody (29270-1-AP)

    IHC staining of human lung using 29270-1-AP (same clone as 29270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29270-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TMEM41B Polyclonal antibody, PBS Only

TMEM41B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29270-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Transmembrane protein 41B, KIAA0033

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29270-1-PBS targets TMEM41B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30810

Product name: Recombinant human TMEM41B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-109 aa of BC035034

Sequence: MAKGRVAERSQLGAHHTTPVGDGAAGTRGLAAPGSRDHQKEKSWVEAGSARMSLLILVSIFLSAAFVMFLVYKNFPQLSEEERVNMKVPRDMDDAKALGKVLSKYKDTF

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 41B
Calculated molecular weight32 kDa
Observed molecular weight22-28 kDa
GenBank accession numberBC035034
Gene Symbol TMEM41B
Gene ID (NCBI) 440026
RRIDAB_2918264
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5BJD5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

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