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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis using 19919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19919-1-AP (TMEM38B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM38B Polyclonal antibody (19919-1-AP)

    WB analysis of MCF-7 using 19919-1-AP

    WB result of TMEM38B antibody (19919-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM38B transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of mouse testis using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TMEM38B Polyclonal antibody (19919-1-AP)

    IHC staining of human kidney using 19919-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19919-1-AP (TMEM38B antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMEM38B Polyclonal antibody (19919-1-AP)

    IF Staining of HepG2 using 19919-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM38B antibody (19919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

TMEM38B Polyclonal antibody

TMEM38B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19919-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C9orf87, D4Ertd89e, TRIC B, TRICB, Trimeric intracellular cation channel type B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19919-1-AP targets TMEM38B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13835

Product name: Recombinant human TMEM38B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 162-291 aa of BC000049

Sequence: ERLVKGDWKPEGDEWLKMSYPAKVTLLGSVIFTFQHTQHLAISKHNLMFLYTIFIVATKITMMTTQTSTMTFAPFEDTLSWMLFGWQQPFSSCEKKSEAKSPSNGVGSLASKPVDVASDNVKKKHTKKNE

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 38B
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight28 kDa
GenBank accession numberBC000049
Gene Symbol TMEM38B
Gene ID (NCBI) 55151
RRIDAB_2878623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM38B encodes endoplasmic reticulum membrane monovalent cation-specific channel which is involved in the release of calcium (Ca2+) from intracellular stores. TMEM38B, also known as TRIC-B, is one of the two trimeric intracellular cation (TRIC) channels. TRIC-B deficiency causes bone disease due to defective Ca2+ release and signaling in the bone cells. (PMID: 34036147, PMID: 39385871)

Protocols

Product Specific Protocols
IF protocol for TMEM38B antibody 19919-1-APDownload protocol
IHC protocol for TMEM38B antibody 19919-1-APDownload protocol
WB protocol for TMEM38B antibody 19919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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