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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM35 Recombinant monoclonal antibody (86724-1-RR)

    WB analysis using 86724-1-RR (same clone as 86724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86724-1-RR (TMEM35 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using TMEM35 Recombinant monoclonal antibody (86724-1-RR)

    IP experiment of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    IP result of anti-TMEM35 (IP:86724-1-RR, 4ug; Detection:86724-1-RR 1:5000) with mouse brain tissue lysate 1960 ug. This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM35 Recombinant monoclonal antibody (86724-1-RR)

    IHC staining of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86724-1-RR (TMEM35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TMEM35 Recombinant monoclonal antibody (86724-1-RR)

    IF Staining of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM35 antibody (86724-1-RR, Clone: 251680G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TMEM35 Recombinant monoclonal antibody (86724-1-RR)

    IF Staining of mouse brain using 86724-1-RR (same clone as 86724-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM35 antibody (86724-1-RR, Clone: 251680G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86724-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86724-1-RR

    Affinity and Kinetic Characterization of 86724-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86724-1-RR against Human TMEM35 were performed. The affinity constant is 2.31 nM.

TMEM35 Recombinant monoclonal antibody, PBS Only

TMEM35 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 86724-1-PBS
Clone No.251680G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

NACHO, Novel acetylcholine receptor chaperone, TMEM35A, transmembrane protein 35

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86724-1-PBS targets TMEM35 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag20589

Product name: Recombinant human TMEM35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 125-167 aa of BC078658

Sequence: LTCRLLIARKPEDRSSEKKPLPGNAEEQPSLYEKAPQGKVKVS

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 35
Calculated molecular weight 167 aa, 18 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC078658
Gene Symbol TMEM35
Gene ID (NCBI) 59353
RRIDAB_3745080
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ53FP2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TMEM35 is a 167-amino acid peptide with a putative NH2 terminal signal peptide, three hydrophobic transmembrane domains, and a nerve growth factor binding domain.TMEM35is 18kDa and has been shown to localize to membrane-bound structures within cells.

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