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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM33 Polyclonal antibody (30639-1-AP)

    WB analysis using 30639-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30639-1-AP (TMEM33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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TMEM33 Polyclonal antibody

TMEM33 Polyclonal Antibody for WB, ELISA
Cat No. 30639-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Transmembrane protein 33, SHINC-3, SHINC3, Protein DB83, DB83

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30639-1-AP targets TMEM33 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM33 fusion protein Ag32368 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 33
Calculated molecular weight 28 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC000948
Gene Symbol TMEM33
Gene ID (NCBI) 55161
RRIDAB_3669743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM33 (transmembrane protein 33), also known as DB83. It is expected to be located in endoplasmic reticulum membrane and nucleus envelope. The protein is widely expressed in prostate cancer and several cancer cell lines (at protein level). And expressed at higher levels in endocrine-resistant breast cancer cells as compared to endocrine-sensitive breast cancer cells. It is also expressed at higher levels in early recurrence breast cancer tissues as compared to non-recurrent breast tumors. The calculated molecular weight of TMEM33 is 28 kDa. It is suggest that TMEM33 has a potency to suppress the membrane-shaping activity of reticulons, thereby regulating the tubular structure of ER (PMID: 25612671).

Protocols

Product Specific Protocols
WB protocol for TMEM33 antibody 30639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols