Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
25507-1-AP targets TMEM24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM24 fusion protein Ag21913 相同性解析による交差性が予測される生物種 |
| Full Name | C2CD2-like |
| Calculated molecular weight | 706 aa, 76 kDa |
| Observed molecular weight | 80-100 kDa |
| GenBank accession number | BC022219 |
| Gene Symbol | C2CD2L |
| Gene ID (NCBI) | 9854 |
| RRID | AB_2880108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14523 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM24, also named as KIAA0285 and C2CD2L, is identified as a pancreatic islet-enriched protein that plays a key role in regulating glucose-sensitive INS release from the reserve pool of granules. This antibody detects TMEM24 protein with MW 70-100 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM24 antibody 25507-1-AP | Download protocol |
| IHC protocol for TMEM24 antibody 25507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis |