Validation Data Gallery
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Tested Applications
| Positive WB detected in | 37°C incubated mouse brain tissue, 37°C incubated rat brain tissue |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31728-1-AP targets TMEM208 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35668 Product name: Recombinant human TMEM208 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 130-173 aa of BC003080 Sequence: PGRALYLLWVNVLGPWFTADSGTPAPEHNEKRQRRQERRQMKRL 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 208 |
| Calculated molecular weight | 173 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC003080 |
| Gene Symbol | TMEM208 |
| Gene ID (NCBI) | 29100 |
| RRID | AB_3742465 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transmembrane protein 208 (TMEM208) can regulate both autophagy and ER stress. When overexpressed, TMEM208 impaired autophagy as characterized by the decrease of the accumulation of LC3-II, decreased degradation of autophagic substrates, and reduced expression of critical effectors and vital molecules of the ER stress and autophagy processes. (PMID: 23691174)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM208 antibody 31728-1-AP | Download protocol |
| WB protocol for TMEM208 antibody 31728-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |