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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM205 Polyclonal antibody (32273-1-AP)

    WB analysis using 32273-1-AP (same clone as 32273-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32273-1-AP (TMEM205 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32273-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TMEM205 Polyclonal antibody (32273-1-AP)

    IF Staining of mouse brain using 32273-1-AP (same clone as 32273-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM205 antibody (32273-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32273-1-PBS in a different storage buffer formulation.

TMEM205 Polyclonal antibody, PBS Only

TMEM205 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 32273-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Transmembrane protein 205, UNQ501/PRO1018

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32273-1-PBS targets TMEM205 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15211

Product name: Recombinant human TMEM205 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-189 aa of BC064948

Sequence: TVNARWLEPRTTAAMWALQTVEKERGLGGEVPGSHQGPDPYRQLREKDPKYSALRQNFFRYHGLSSLCNLGCVLSNGLCLAGLALEIRSL

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 205
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC064948
Gene Symbol TMEM205
Gene ID (NCBI) 374882
RRIDAB_3742561
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6UW68
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

As a member of the transmembrane proteins (TMEMs), TMEM205 constructed transmembrane channels for a variety of substances, mediating communication between the intracellular and extracellular environment (PMID: 38850316).

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