Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25489-1-AP targets TMEM199 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22009 Product name: Recombinant human TMEM199 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-208 aa of BC033113 Sequence: MASSLLAGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSLKALVITIFNFIVTVVAAFVCTYLGSQYIFTEMASRVLAALIVASVVGLAELYVMVRAMEGELGEL 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 199 |
| Calculated molecular weight | 208 aa, 23 kDa |
| Observed molecular weight | 18-23 kDa |
| GenBank accession number | BC033113 |
| Gene Symbol | TMEM199 |
| Gene ID (NCBI) | 147007 |
| RRID | AB_2880101 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N511 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM199, also named as C17orf32, is a component of the complex sense antisense architecture (CSAGA).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM199 antibody 25489-1-AP | Download protocol |
| WB protocol for TMEM199 antibody 25489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |