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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of HepG2 using 21355-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of L02 using 21355-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of MCF-7 using 21355-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of mouse liver using 21355-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver cancer using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

TMEM195 Polyclonal antibody

TMEM195 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21355-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21355-1-AP targets TMEM195 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15774

Product name: Recombinant human TMEM195 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-333 aa of BC108676

Sequence: HRMAHEVNIMWAGHQTHHSSEDYNLSTALRQSVLQIYTSWIFYSPLALFIPPSVYAVHLQFNLLYQFWIHTEVINNLGPLELILNTPSHHRVHHGRNRYCIDKNYAGVLIIWDKIFGTFEAENEKVVYGLTHPINTFEPIKVQFHHLFSIWTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQ

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 195
Calculated molecular weight 445 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC108676
Gene Symbol TMEM195
Gene ID (NCBI) 392636
RRIDAB_2878845
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM195 antibody 21355-1-APDownload protocol
IHC protocol for TMEM195 antibody 21355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Infect Dis

Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.

Authors - Katrin Watschinger
View Article
humanWB

Hum Genet

Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.

Authors - Volkan Okur
View Article