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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMEM195 Polyclonal antibody

TMEM195 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21355-1-AP

Synonyms

Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 21355-1-AP

    WB analysis of HepG2 using 21355-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 21355-1-AP

    WB analysis of L02 using 21355-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 21355-1-AP

    WB analysis of MCF-7 using 21355-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 21355-1-AP

    WB analysis of mouse liver using 21355-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21355-1-AP

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21355-1-AP

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human liver cancer using 21355-1-AP

    IHC staining of human liver cancer using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21355-1-AP is TMEM195 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM195 fusion protein Ag15774 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 195
Calculated molecular weight 445 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC108676
Gene symbol TMEM195
Gene ID (NCBI) 392636
RRIDAB_2878845
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM195 antibody 21355-1-APDownload protocol
IHC protocol for TMEM195 antibody 21355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Infect Dis

Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.

Authors - Katrin Watschinger
View Article
humanWB

Hum Genet

Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.

Authors - Volkan Okur
View Article