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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of HepG2 using 21355-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of L02 using 21355-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of MCF-7 using 21355-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    WB analysis of mouse liver using 21355-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM195 Polyclonal antibody (21355-1-AP)

    IHC staining of human liver cancer using 21355-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

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TMEM195 Polyclonal antibody

TMEM195 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21355-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21355-1-AP targets TMEM195 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM195 fusion protein Ag15774 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 195
Calculated molecular weight 445 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC108676
Gene Symbol TMEM195
Gene ID (NCBI) 392636
RRIDAB_2878845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM195 antibody 21355-1-APDownload protocol
IHC protocol for TMEM195 antibody 21355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Infect Dis

Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.

Authors - Katrin Watschinger
View Article
humanWB

Hum Genet

Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.

Authors - Volkan Okur
View Article