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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using TMEM189 Polyclonal antibody (31293-1-AP)

    IHC staining of human colon using 31293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31293-1-AP (TMEM189 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using TMEM189 Polyclonal antibody (31293-1-AP)

    IHC staining of human colon using 31293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31293-1-AP (TMEM189 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM189 Polyclonal antibody

TMEM189 Polyclonal Antibody for IHC, ELISA
Cat No. 31293-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Transmembrane protein 189, Plasmanylethanolamine desaturase 1, PEDS1, PDES, KUA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31293-1-AP targets TMEM189 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34323

Product name: Recombinant human TMEM189 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 182-270 aa of BC142966

Sequence: TNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQKIK

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 189
Observed molecular weight31 kDa
GenBank accession numberBC142966
Gene Symbol TMEM189
Gene ID (NCBI) 387521
RRIDAB_3742416
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDA5PLL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM189 (Plasmanylethanolamine desaturase 1), also known as PEDS1. It is predicted to be located in the endoplasmic reticulum membrane. Plasmalogens are glycerophospholipids with a hydrocarbon chain linked by a vinyl ether bond at the glycerol sn-1 position, and are involved in antioxidative and signaling mechanisms (PMID: 31604315). The molecular weight of TMEM189 is 31 kDa.

Protocols

Product Specific Protocols
IHC protocol for TMEM189 antibody 31293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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