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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using TMEM189 Polyclonal antibody (31293-1-AP)

    IHC staining of human colon using 31293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31293-1-AP (TMEM189 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using TMEM189 Polyclonal antibody (31293-1-AP)

    IHC staining of human colon using 31293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31293-1-AP (TMEM189 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TMEM189 Polyclonal antibody

TMEM189 Polyclonal Antibody for IHC, ELISA
Cat No. 31293-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

Transmembrane protein 189, Plasmanylethanolamine desaturase 1, PEDS1, PDES, KUA

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31293-1-AP targets TMEM189 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34323

Product name: Recombinant human TMEM189 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 182-270 aa of BC142966

Sequence: TNQIHKWSHTYFGLPRWVTLLQDWHVILPRKHHRIHHVSPHETYFCITTGWLNYPLEKIGFWRRLEDLIQGLTGEKPRADDMKWAQKIK

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 189
Observed molecular weight31 kDa
GenBank accession numberBC142966
Gene Symbol TMEM189
Gene ID (NCBI) 387521
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDA5PLL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM189 (Plasmanylethanolamine desaturase 1), also known as PEDS1. It is predicted to be located in the endoplasmic reticulum membrane. Plasmalogens are glycerophospholipids with a hydrocarbon chain linked by a vinyl ether bond at the glycerol sn-1 position, and are involved in antioxidative and signaling mechanisms (PMID: 31604315). The molecular weight of TMEM189 is 31 kDa.

Protocols

Product Specific Protocols
IHC protocol for TMEM189 antibody 31293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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