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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse brain using 28031-1-AP (same clone as 28031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse brain using 28031-1-AP (same clone as 28031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse cerebellum using 28031-1-AP (same clone as 28031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28031-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TMEM184B Polyclonal antibody (28031-1-AP)

    IHC staining of mouse cerebellum using 28031-1-AP (same clone as 28031-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28031-1-AP (TMEM184B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28031-1-PBS in a different storage buffer formulation.

TMEM184B Polyclonal antibody, PBS Only

TMEM184B Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 28031-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28031-1-PBS targets TMEM184B in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27704

Product name: Recombinant human TMEM184B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 318-407 aa of BC015489

Sequence: VYADKRLDAQGRCAPMKSISSSLKETMNPHDIVQDAIHNFSPAYQQYTQQSTLEPGPTWRGGAHGLSRSHSLSGARDNEKTLLLSSDDEF

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 184B
GenBank accession numberBC015489
Gene Symbol TMEM184B
Gene ID (NCBI) 25829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y519
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transmembrane protein 184B (TMEM184B) is a seven-pass transmembrane protein highly expressed in the nervous system and is thought to play a role in neuronal excitability, synaptic structure, and expression of key developmental and adult pathways involved in neuronal differentiation (PMID: 27122027; 37730546). TMEM184B may activate the MAP kinase signaling pathway (PMID: 12761501). TMEM184B has also been reported to be necessary for interleukin-31-induced itch, promoting adult somatosensation through developmental Wnt signaling and promotion of proper pruriceptive gene expression (PMID: 34629389).