Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25366-1-AP targets TMEM182 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TMEM182 fusion protein Ag21906 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 182 |
| Calculated molecular weight | 229 aa, 26 kDa |
| Observed molecular weight | 15 kDa 26 kDa |
| GenBank accession number | BC130308 |
| Gene Symbol | TMEM182 |
| Gene ID (NCBI) | 130827 |
| RRID | AB_2880046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZP80 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM182 antibody 25366-1-AP | Download protocol |
| IHC protocol for TMEM182 antibody 25366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB Bioadv TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression |