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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    WB analysis of mouse liver using 24799-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24799-1-AP (TMEM179 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TMEM179 Polyclonal antibody (24799-1-AP)

    IHC staining of human breast cancer using 24799-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24799-1-AP (TMEM179 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

TMEM179 Polyclonal antibody

TMEM179 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24799-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

C14orf90, TMEM179, TMEM179A, transmembrane protein 179, Transmembrane protein 179A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24799-1-AP targets TMEM179 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20502

Product name: Recombinant human TMEM179 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 115-197 aa of BC148844

Sequence: AFVVFLVFIASTIVSVGFTMWCDTITEKGTVPHSCEELQDIDLELGVDNSAFYDQFAIAQVGGSGQEGRLAMLGGGHLLLDIC

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 179
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC148844
Gene Symbol TMEM179
Gene ID (NCBI) 388021
RRIDAB_2879732
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZVK1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TMEM179 antibody 24799-1-APDownload protocol
IHC protocol for TMEM179 antibody 24799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Ecotoxicol Environ Saf

NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

Authors - Zhixin He
  • KD Validated
View Article
humanWB

PLoS One

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.

Authors - Kui Xiao
View Article