Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
31210-1-PBS targets TMEM174 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35041 Product name: Recombinant human TMEM174 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-243 aa of BC019346 Sequence: MEQGSGRLEDFPVNVFSVTPYTPSTADIQVSDDDKAGATLLFSGIFLGLVGITFTVMGWIKYQGVSHFEWTQLLGPVLLSVGVTFILIAVCKFKMLSCQLCKESEERVPDSEQTPGGPSFVFTGINQPITFHGATVVQYIPPPYGSPEPMGINTSYLQSVVSPCGLITSGGAAAAMSSPPQYYTIYPQDNSAFVVDEGCLSFTDGGNHRPNPDVDQLEETQLEEEACACFSPPPYEEIYSLPR 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 174 |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC019346 |
| Gene Symbol | TMEM174 |
| Gene ID (NCBI) | 134288 |
| RRID | AB_3742409 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WUU8 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Transmembrane protein 174 (TMEM174) is comprised of 243 amino acids, and contains two predicted transmembrane helices which determine its subcellular localization in endoplasmic reticulum and influences its functions (PMID: 20331980). TMEM174) mRNA is easily detectable in human kidney tissues and activates AP-1 and promotes 293T cell proliferation (PMID: 25202393). The Tmem174 protein was extremely highly expressed in the kidney and localized at the apical membrane of the renal proximal tubules. Tmem174 binds to NaPi2a on the cell membrane and is involved in the internalization of NaPi2a (PMID: 35428804). Tmem174 is considered to be a component of the NaPi2a/NHERF1 complex that receives signals from PTH and FGF23 (PMID: 35428804). This antibody may recognize the dimer.