Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroIF/ICCAffinity

WB analysis using 80165-1-RR (same clone as 80165-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80165-1-RR (TMEM173/STING antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 80165-1-RR (same clone as 80165-1-PBS)
IP result of anti-TMEM173/STING (IP: 80165-1-RR, 4ug; Detection: 80165-1-RR 1:8000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80165-1-RR (same clone as 80165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80165-1-RR (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IF Staining of mouse cerebellum using 80165-1-RR (same clone as 80165-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
IF Staining of THP-1 using 80165-1-RR (same clone as 80165-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using TMEM173/STING antibody (80165-1-RR, Clone: 7K7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80165-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80165-1-RR
Biolayer interferometry (BLl) kinetic assays of 80165-1-RR against Human TMEM173/STING were performed. The affinity constant is 0.776 nM.

TMEM173/STING Recombinant monoclonal antibody, PBS Only

TMEM173/STING Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

Cat No. 80165-1-PBS

Clone No.7K7

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

STING, 7K7, Endoplasmic reticulum interferon stimulator, ERIS, MITA

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Published Applications

IF	See 1 publications below

Product Information

80165-1-PBS targets TMEM173/STING in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag13921 Product name: Recombinant human TMEM173 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 174-379 aa of BC047779 Sequence: ELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS 相同性解析による交差性が予測される生物種
Full Name	transmembrane protein 173
Calculated molecular weight	379 aa, 42 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC047779
Gene Symbol	STING
Gene ID (NCBI)	340061
RRID	AB_2918870
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q86WV6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Publications

Species	Application	Title
	IF	FASEB J STING Facilitates Vascular Calcification via p-STAT1/NLRP3 Signal Authors - Lihe Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 80165-1-RR (same clone as 80165-1-PBS)

IP experiment of HeLa using 80165-1-RR (same clone as 80165-1-PBS)

IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

IHC staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

IHC staining of human colon using 80165-1-RR (same clone as 80165-1-PBS)

IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

IHC staining of human liver using 80165-1-RR (same clone as 80165-1-PBS)

IF Staining of human tonsillitis using 80165-1-RR (same clone as 80165-1-PBS)

IF Staining of mouse cerebellum using 80165-1-RR (same clone as 80165-1-PBS)

IF Staining of THP-1 using 80165-1-RR (same clone as 80165-1-PBS)

Affinity and Kinetic Characterization of 80165-1-RR