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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TMEM16A/DOG1 Polyclonal antibody (33212-1-AP)

    IHC staining of human lung cancer using 33212-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 33212-1-AP (TMEM16A/DOG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TMEM16A/DOG1 Polyclonal antibody (33212-1-AP)

    IHC staining of human lung cancer using 33212-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 33212-1-AP (TMEM16A/DOG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TMEM16A/DOG1 Polyclonal antibody

TMEM16A/DOG1 Polyclonal Antibody for IHC, ELISA
Cat No. 33212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

ANO1, Anoctamin 1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, DOG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33212-1-AP targets TMEM16A/DOG1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM16A/DOG1 fusion protein Ag37099 相同性解析による交差性が予測される生物種
Full Name anoctamin 1, calcium activated chloride channel
Calculated molecular weight114kDa，986aa
GenBank accession numberNM_018043.5
Gene Symbol TMEM16A
Gene ID (NCBI) 55107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5XXA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANO1 also named as DOG1, ORAOV2, TAOS2 and TMEM16A, acts as a calcium-activated chloride channel. It is required for normal tracheal development. ANO1 (or DOG1) is used as an aid in the identification and diagnosis of gastrointestinal stromal tumors (GIST) within the context of an antibody panel, the patient's clinical history, and other diagnostic tests evaluated by a qualified pathologist.

Protocols

Product Specific Protocols
IHC protocol for TMEM16A/DOG1 antibody 33212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols