Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag14362 Product name: Recombinant human TMEM165 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 61-177 aa of BC003545 Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 165 |
| Calculated molecular weight | 324 aa, 35 kDa |
| GenBank accession number | BC003545 |
| Gene Symbol | TMEM165 |
| Gene ID (NCBI) | 55858 |
| RRID | AB_3670844 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HC07 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TMEM165 antibody 83146-1-RR | Download protocol |
| IF protocol for TMEM165 antibody 83146-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
