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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of LNCaP using 68584-1-Ig (same clone as 68584-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68584-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of K-562 using 68584-1-Ig (same clone as 68584-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68584-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis of U-251 using 68584-1-Ig (same clone as 68584-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68584-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TMEM165 Monoclonal antibody (68584-1-Ig)

    WB analysis using 68584-1-Ig (same clone as 68584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68584-1-Ig (TMEM165 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68584-1-PBS in a different storage buffer formulation.

TMEM165 Monoclonal antibody, PBS Only

TMEM165 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68584-1-PBS
Clone No.3B11C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68584-1-PBS targets TMEM165 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14872

Product name: Recombinant human TMEM165 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 61-177 aa of BC003545

Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 165
Calculated molecular weight 324 aa, 35 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC003545
Gene Symbol TMEM165
Gene ID (NCBI) 55858
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9HC07
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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