Validation Data Gallery
Tested Applications
| Positive WB detected in | HH cells, HepG2 cells, Jurkat cells, Ramos cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31917-1-AP targets TMEM143 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36277 Product name: Recombinant human TMEM143 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 51-200 aa of BC016919 Sequence: MGEYRKMWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYVYIH 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 143 |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 45-52 kDa |
| GenBank accession number | BC016919 |
| Gene Symbol | TMEM143 |
| Gene ID (NCBI) | 55260 |
| RRID | AB_3670142 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96AN5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transmembrane protein 143 (TMEM143) is a transmembrane protein and a member of the TMEM (Transmembrane) protein family, which is located in mitochondria (PMID: 11256614). It is known that members of the TMEM family play an important role in the development and metastasis of cancer, especially in conveying information between the extracellular environment and cytoplasmic proteins (PMID: 31454669).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM143 antibody 31917-1-AP | Download protocol |
| WB protocol for TMEM143 antibody 31917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |