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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM143 Polyclonal antibody (31917-1-AP)

    WB analysis using 31917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31917-1-AP (TMEM143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TMEM143 Polyclonal antibody (31917-1-AP)

    IHC staining of human placenta using 31917-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TMEM143 Polyclonal antibody (31917-1-AP)

    IHC staining of human placenta using 31917-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31917-1-AP (TMEM143 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TMEM143 Polyclonal antibody

TMEM143 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31917-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

UNQ5922/PRO19813, Transmembrane protein 143

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHH cells, HepG2 cells, Jurkat cells, Ramos cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31917-1-AP targets TMEM143 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM143 fusion protein Ag36277 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 143
Calculated molecular weight52 kDa
Observed molecular weight45-52 kDa
GenBank accession numberBC016919
Gene Symbol TMEM143
Gene ID (NCBI) 55260
RRIDAB_3670142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96AN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transmembrane protein 143 (TMEM143) is a transmembrane protein and a member of the TMEM (Transmembrane) protein family, which is located in mitochondria (PMID: 11256614). It is known that members of the TMEM family play an important role in the development and metastasis of cancer, especially in conveying information between the extracellular environment and cytoplasmic proteins (PMID: 31454669).

Protocols

Product Specific Protocols
WB protocol for TMEM143 antibody 31917-1-APDownload protocol
IHC protocol for TMEM143 antibody 31917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols