Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31327-1-AP targets TMEM135 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34057 Product name: Recombinant human TMEM135 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 352-458 aa of BC051462 Sequence: YLASKLVETMYFKGIEAGKVPYFPHADTIIYSISTAICFQAAVMEVQTLRPSYWKFLLRLTKGKFAVMNRKVLDVFGTGASKHFQDFIPRLDPRYTTVTPELPTEFS 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 135 |
| Calculated molecular weight | 458 aa, 52 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC051462 |
| Gene Symbol | TMEM135 |
| Gene ID (NCBI) | 65084 |
| RRID | AB_3669944 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMEM135, also known as PMP52, contains 3 N-terminal transmembrane domains and 4 C-terminal transmembrane domains. TMEM135 is up-regulated in bone marrow-derived mesenchymal stem cells during differentiation into adipocytes compared to osteoblasts(PMID: 18637193).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM135 antibody 31327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |