Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat spleen tissue, mouse spleen tissue |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25078-1-AP targets TMEM134 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18533 Product name: Recombinant human TMEM134 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-124 aa of BC125134 Sequence: QFSIDDAFELSLEDGGPGPESSGVARFGPLHFERRARFEVADEDKQSRLRYQNLENDEDGAQASPEPDGGVGTRDSSRTSIRSSQWSFSTISSSTQRSYNTCCSWTQHPLIQKNRRVV 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 134 |
| Calculated molecular weight | 180 aa, 20 kDa |
| Observed molecular weight | 22-27 kDa |
| GenBank accession number | BC125134 |
| Gene Symbol | TMEM134 |
| Gene ID (NCBI) | 80194 |
| RRID | AB_3669461 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6X4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transmembrane protein 134 is a protein encoded by the TMEM134 gene. TMEM134 is regulated by the promoter GXP50275. TMEM134 is ubiquitously expressed in the majority of human tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMEM134 antibody 25078-1-AP | Download protocol |
| WB protocol for TMEM134 antibody 25078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |