Validation Data Gallery
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Tested Applications
| Positive WB detected in | HaCaT cells, fetal human brain tissue, MCF-7 cells, SH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
Product Information
25301-1-AP targets TMEM132A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18142 Product name: Recombinant human TMEM132A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 636-850 aa of BC114569 Sequence: VLELRVQPVMGISLTLSRGTAHPGEVTATCWAQSALPAPKQEVALSLWLSFSDHTVAPAELYDRRDLGLSVSAEEPGAILPAEEQGAQLGVVVSGAGAEGLPLHVALHPPEPCRRGRHRVPLASGTAWLGLPPASTPAPALPSSPAWSPPATEATMGGKRQVAGSVGGNTGVRGKFERAEEEARKEETEAREEEEEEEEEMVPAPQHVTELELGM 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane protein 132A |
| Calculated molecular weight | 1023 aa, 110 kDa |
| Observed molecular weight | 100-140 kDa |
| GenBank accession number | BC114569 |
| Gene Symbol | TMEM132A |
| Gene ID (NCBI) | 54972 |
| RRID | AB_2880018 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q24JP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM132A antibody 25301-1-AP | Download protocol |
| IHC protocol for TMEM132A antibody 25301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol TMEM132A, a Novel Wnt Signaling Pathway Regulator Through Wntless (WLS) Interaction.
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Development TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration
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