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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IP experiment of HeLa using 23142-1-AP

    IP result of anti-TMEM127 (IP:23142-1-AP, 3ug; Detection:23142-1-AP 1:500) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human heart using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IF Staining of NIH/3T3 using 23142-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TMEM127 antibody (23142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TMEM127 Polyclonal antibody

TMEM127 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23142-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Transmembrane protein 127

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

23142-1-AP targets TMEM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM127 fusion protein Ag19472 相同性解析による交差性が予測される生物種
Full Name transmembrane protein 127
Calculated molecular weight 238 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC039892
Gene Symbol TMEM127
Gene ID (NCBI) 55654
RRIDAB_2879217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO75204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TMEM127 antibody 23142-1-APDownload protocol
IF protocol for TMEM127 antibody 23142-1-APDownload protocol
IP protocol for TMEM127 antibody 23142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article
humanWB

Tissue Cell

Exosomes from adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation

Authors - Xiaoyan Hao
View Article