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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IP experiment of HeLa using 23142-1-AP

    IP result of anti-TMEM127 (IP:23142-1-AP, 3ug; Detection:23142-1-AP 1:500) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human stomach cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human heart using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM127 Polyclonal antibody (23142-1-AP)

    IHC staining of human liver cancer using 23142-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TMEM127 Polyclonal antibody (23142-1-AP)

    IF Staining of NIH/3T3 using 23142-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TMEM127 antibody (23142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TMEM127 Polyclonal antibody

TMEM127 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 23142-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Transmembrane protein 127

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

23142-1-AP targets TMEM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19472

Product name: Recombinant human TMEM127 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 139-238 aa of BC039892

Sequence: QCATVIGFSYWASELILAQQQQHKKYHGSQVYVTFAVSFYLVAGAGGASILATAANLLRHYPTEEEEQALELLSEMEENEPYPAEYEVINQFQPPPAYTP

相同性解析による交差性が予測される生物種
Full Name transmembrane protein 127
Calculated molecular weight 238 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC039892
Gene Symbol TMEM127
Gene ID (NCBI) 55654
RRIDAB_2879217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO75204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TMEM127 antibody 23142-1-APDownload protocol
IF protocol for TMEM127 antibody 23142-1-APDownload protocol
IP protocol for TMEM127 antibody 23142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article
humanWB

Tissue Cell

Exosomes from adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation

Authors - Xiaoyan Hao
View Article